Reference no: EM13145399

1. You have discovered a C. elegans mutant hermaphrodite with funky movement. You call it fnk-1. The fnk-1 mutations behaves as a recessive mutation. The mutation causing this phenotype maps to chromosome II, not the X. You wonder whether genes that affect muscle development or muscle function are clustered together, near your gene, and attempt to answer this question by trying to isolate additional mutations that give the same funky movement in the region of chromosome II, near your fnk-1 gene.
A. How would you do such a screen ?
B. How would you know when to stop looking, when have you « saturated » the region ?

2. You tell a friend about this funky moving worm phenotype and your friend also a C. elegans geneticist tells you that he has a strain with a similar phenotype, let's call this one fnk-2. How could you test if the second mutation, fnk-2, is a mutation in your same original gene, fnk-1?