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A standard procedure in pufying tubulin is to extract the tissue on ice, then add GTP and warm the tissue extract to 37C. The warmed extract is then centrifuged at a speed insufficient to pellet average sized proteins, but sufficient to pellet large protein complexes. After centrifugation, the supernant is discarded, and the solution is agian warmed for a few minutes. Then centrifugation at the same speed described above is again performed. This process is repeated several times.
How would this processs result in purification of tubulin?
What contaminating proteins would likely co-purify with the tubulin?
X-linked Recessive: colour-blindness, malepatterned baldness, hemophilia Color-blindness is caused by a recessive gene (b) on X chromosome. Predict phenotypes for children of a female carrier with a normal male. A genetic wasteland.
Describe the relationship between biological psychology and other fields in psychology and neuroscience.
Mrs. Smith and Mrs. Jones had baby girls same day in same hospital. Mrs. Smith named her baby Shirley and Mrs. Jones named her baby Jane.
How a biologist would explain how ability to run fast evolved in cheetahs, assume their ancestors would only run 20 miles per hour. How various total depurinations in your body each day.
Modern theory suggests that the early pre-life atmosphere on Earth was a reducing one. Why is it believed that oxygen was not present as life formed on Earth.
Why were the ratios the similar in the first part but different in Part 2.
Schupack attempted to exercise right in first refusal McDonalds would not recognize the right in Schupack, claiming that it was personal to Copeland and so non-assignable without their consent. Schupack brought an action for specific performance, req..
Fluorescently labeled annexin V is mixed with each vesicle preparation. The vesicles are washed to remove unbound annexin V and next examined by fluorescence microscopy. Presence of annexin V is detected by green fluorescence, as shown above. What ca..
Muscle cells, while an individual is exercising heavily and at what time the muscle becomes oxygen deprived, convert pyruvate to lactate. What happens to the lactate in skeletal muscle cells.
Frank found himself in the midst of an ethical dilemma at work, and was using an ethical decision-making matrix, to address his work and personal values. Frank was most likely in which phase of the Ethical Decision-Making Framework.
Swelling of the abdomen and legs due to edema is characteristic of starving children with kwashiorkor, protein-deficiency disorder. What is the relationship between this symptom and procedure of capillary exchange.
Across between short-tailed mouse and a long-tailed mouse yields all short-tailed mice. Crosses among the F1 yield an F2 of 48 short-tailed and 22 long-tailed. Suggest the model to explain and perform a chi-square test of your results.
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