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1. How can we make an antibody to a specific protein?2. In what ways can this antibody be used as a molecular tool in studying this protein?3. Explain the difference between an allosteric and competitive inhibitor.4. What are the four levels of protein organization? Explain them.
5. In SDS-Page, proteins are separated by length. If the same length proteins can fold into loose (bigger) or condensed (smaller) shapes and can even complex with other proteins, how are individual proteins separated solely according to their length? Additionally, proteins can have different charges. Proteins with more basic amino acids have a greater negative charge while proteins with more acidic amino acids have a greater positive charge. Proteins travel from the negative electrode to positive electrode in SDS-Page. How does their intrinsic charge not affect their migration (true for most proteins)?
Assume you have an unknown DNA that you know is phage DNA - It is either T7 or lambda. You perform a restriction enzyme digest and run the results on a gel.
Discuss and explain how ideas about stress immunization evolved into realization that maternal behavior actually plays an important role in mediating the effect of early stress, citing both animal and human research.
As a pea plant grows, its root cells divide by mitosis. If the diploid chromosome number of the plant is fourteen, how various chromosomes will each newly formed root cell have.
Suppose that you discover a new life form on Mars. These organisms have DNA containing the same four nucleotides as Earth DNA. They also make proteins using a triplet code, so they use the same 64 codons that Earth organisms do.
What is the maximum theoretical heart rate based on this information? Cardiac muscle cells are capable of grade.
What could be expected phenotypes, and in what number will the y be expected? Suppose no linkage.
Black coat of animal is dominant; white is the alternative recessive. While true-breeding black is crossed to true-breeding white, what is the possibility that a black F2 is heterozygous.
For a given reaction, G = -4 kcal/mole and the energy of activation for the uncatalyzed reaction = 5 kcal/mole. You add an enzyme, after which the activation energy decreased to 3 kcal/mole.
In a 1973-74 situation comedy All in the Family, the character Archie Bunker says, Let me tell you something about beer, You can never buy beer, you can only rent it.
What type of compensation plan will you recommend? What are some of the problems you want to be aware of. Develop new coating methods and new applications of coating.
When Mexican hairless dogs are crosses with normally haired dogs, about half the pups are hairless and half have hair. When, however, two Mexican hairless dogs are mated, about a third of the pups produced have hair, about two thirds are hairless, an..
Suppose a cross has been made with maize between a plant with colored, nonshrunken kernals (C/C Sh/Sh) and a plant with colorless, shrunken kernels (c/c sh/sh).
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