How to destabilize the polymerase are possible explanations

Reference no: EM13146907

An experiment was conducted to determine the hairpin loop region in termination. Two DNA templates were prepared and used in in vitro transcription assay. The samples were analyzed for release of the RNA polymerase.
Construct 1 - hairpin sequence removed; Construct 2 - two copies of hairpin sequence inserted; Construct 3 - normal DNA with only one hairpin sequence

Construct Level of truncated Nascent
RNA released (arbitrary units)
1 10
2 11
3 10

The data from this experiment suggest that
A. hairpin loops are not required for termination.
B. two hairpin loops rapidly destabilize the polymerase.
C. the data are inconclusive.
D. additional hairpin loops increase the stability of the RNA:DNA hybrid.
E. both hairpin loops are not required for termination and two hairpin loops rapidly destabilize the polymerase are possible explanations.

Reference no: EM13146907

Questions Cloud

Name the three major modifications of mrna of eukaryotes : Name the three major modifications of mRNA of eukaryotes before it is transported to the cytoplasm. In addition, tell why each of the modifications is necessary.

Compute the freezing point : Calculate the freezing point of the following solutions, assuming complete dissociation. Part A= 0.114mK2S Part B=20.5g of CuCl2 in 401g water Part C=6.5% NaNO3 by mass

What will be the incidence of cystic fibrosis in your island : What will be the incidence of cystic fibrosis in your island?Is this population in Hardy Weinberg equilibrium at the start?

What is the ksp for caf2 : A saturated solution of CaF2 had a calcium ion concentration of 2.20 x 10^-4 M. What is the Ksp for CaF2?

How to destabilize the polymerase are possible explanations : An experiment was conducted to determine the hairpin loop region in termination. Two DNA templates were prepared and used in in vitro transcription assay. The samples were analyzed for release of the RNA polymerase.

Cost of executing a transactiion is much lower firms : the cost of executing a transactiion is much lower firms have the ability to gather useful information about buyers firms can reduce their reaction times to changing market conditions and increase their sales reach.

What was the molarity of the aqueous acid solution : By titration, 23.4 mL of aqueous H2SO4 neutralized 24.8 mL of 0.0495 M LiOH solution. What was the molarity of the aqueous acid solution?

Jnoes was knocked down by a car driven : In May 2013, Jnoes was knocked down by a car driven by Ruth. As a result of the accident Jones was not able to continue his work and had to undergo extensive medical care and physiotherapy. He sued Ruth for loss of earning capacity, non pecunia..

Non-controlling interest share of consolidated net income : Race decided to use the equity method to account for this investment. What was the non-controlling interest's share of consolidated net income?

User Account

All Pages