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Bacteria that have been exposed to two different bacterial strains: one carries only a gene for resistance to the antibiotic Ampicillin; the other carries the gene for Ampicillin resistance as well as a gene for green fluorescent protein (GFP), which glows under UV light. Some bacteria took up the Ampicillin-resistance gene, others took up the genes for Ampicillin resistance and for GFP, but some took up neither (non-transformed). The bacteria were then spread on petri dishes with either nutrients and Ampicillin or just nutrients.
Why do you plate only a small amount of the bacterial solution on the Ampicillin-treated plates, and an even smaller amount on the plates without Ampicillin, when you are trying to calculate transformation efficiency.
Explain the role of the citric acid cycle as a central metabolic mechanism. Explain what happens to the cells' abilities to oxidize acetyl CoA when intermediates of the cycle are drained off for amino acid biosynthesis.
How organismal respiration and cellular respiration are interconnected. While you visit him, his teeth are chattering, his skin is cool and clammy to touch, and he complains of feeling cold even though his room is quite warm. Elucidate his symptoms.
What is bioinformatics, and why is this discipline essential for studying genomes? Provide two examples of bioinformatics applications.
Sort the statements into appropriate bins depending on which type of learning they explain. What is her anatomical dead space volume.
Are fungi gram positive or gram negative? Explain based on the structure of its cellular envelope and explain the basis for your answer in terms of specific non-informational macromolecules.
A plasmid contains genes for ampicillin resistance and tetracycline resistance and has single sites for restriction sites BglII, PstI and EcoRI. When genes are inserted into the PstI site, transformants are resistant only to ampicillin. In which r..
Examination of a large sample of individuals from the population reveals there is six times as many heterozygote as homozygote recessive individuals in this populaion. What is the frequency of the recessive allele?
The brown and scarlet mutations assort independently. If fruit flies that are heterozygous for both of these mutations are intercrossed, what sorts of progeny will they create, and in what proportions? Describe how you got the answer.
You're an international athlete. For breakfast you had a lemon poppyseed muffin. Now theres a knock at your door. It is the internation no drugs in athletes committee. They want a sample of your urine for a drug test.
Ethanol is supplied in a 95 percent solution. You need 50mL of 70 percent Ethanol for your experiment. How would you make this solution?
The big majority of the frogs in this population are green. A few individual frogs carry a mutation that prevents the production of the green pigment.
The female I-1 and her mate, male I-2, had four children, one of whom has albinism. What is the probability that they could have had a total of four children
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