Reference no: EM131128681

for the attached values, explain how you would prepare the following solution.

a. How to calculate much BSA and how much buffer to use in the experiment.

b. You are provided with 10 mLs of 10.00 mg/mL BSA solution in 50 mM phosphate buffer pH 7.0, 100 mLs of 50 mM phosphate buffer pH 7.0, a 20-200 µL pipetman, a 200-1000 µL pipetman, a sufficient supply of the appropriate pipet tips, and twenty 5-mL test tubes.

Explain how you would prepare the following solutions to construction your BSA standard curve.

1.0 mL of 100 µg/mL BSA (in 50 mM phosphate buffer pH 7.0)

1.0 mL of 200 µg/mL BSA (in 50 mM phosphate buffer pH 7.0)

1.0 mL of 350 µg/mL BSA (in 50 mM phosphate buffer pH 7.0)

1.0 mL of 500 µg/mL BSA (in 50 mM phosphate buffer pH 7.0)

1.0 mL of 700 µg/mL BSA (in 50 mM phosphate buffer pH 7.0)

1.0 mL of 900 µg/mL BSA (in 50 mM phosphate buffer pH 7.0)

1.0 mL of 1.2 mg/mL BSA (in 50 mM phosphate buffer pH 7.0)