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Q1. At the same time that you did the viable cell count of your bacterial culture (in problem 10), you also counted the cells in a Petroff-Hausser chamber; however, the cell count you obtained from Petroff-Hausser chamber is nearly double that obtained in the viable cell count. Based on this result, what may you conclude about the growth phase of the bacterial culture you are working with - was it likely to be in lag phase, exponential or stationary phase. Explain the answer.
Q2. In ward's Ap biology lab 7 (the drosophila lab) describe the parental cross you received; use genetic symbols. Depict a punnett square to show how the possible allelic combinations for this gene in the F1 generation.
A cell whose cytoplasm has a concentration of 0.02 molar glucose is placed in a test tube of water containing 0.02 molar glucose. Assume that glucose is not actively transported into the cell, which terms describes the tonicity of an external solutio..
After a long scuba diving session on a Caribbean reef, David boards a plane to Denver. He begins to feel pain in his elbows on a flight back home to Denver.
Results of this analysis show that one of the most rapidly reassociating classes of DNA is substantially reduced in the older individual with respect to the 2 year-old. How can you describe this finding.
Suppose the diameer of a neutral helium atom is 1.40 x 10 to the second power pm. supposed that we could line up helium atoms side by side in contact with one another. How numerous atoms would it take to make the distance from end to end 9.00cm.
Elucidate results of all four crosses, using clearly defined gene symbols. describe origin of both of the original prototrophic colonies.
List all probable points at which caffeine may be exerting its effect. Caffeine is known to alter the transmembrane movement of calcium. What is (are) the possible site(s) at which caffeine is acting.
Work out this chi-square value. How to do a Chi Square trouble for the gentics class.
If you transform bacteria with the plasmid library made in part b above, divide the transformatnts into 20 aliquots and find one has the insert of interest, now how many clones do you need to screen.
Which distributed database process is best under these circumstances. How is the replication of the viral genome of retroviruses unique among the viruses? Why was it before believed that only DNA viruses could cause cancer.
Diplomonads, such as Giardia contain two haploid nuclei per trophozoite. Thus, During the G1 phase of the cell cycle, there should be a total of how several unreplicated chromosomes per trophozoite, and throughout the G2 phase, how many replicated ch..
If you were asked to create a karyotype from the chromosome spreads you made from HeLa cells in Activity 4.2.1, list materials you would use and explain how you would make the karyotype.
The membrane vesicles are next washed, the membranes solubilized and the proteins denatured and analyzed by SDS gel electrophoresis and Western blotting using polyclonal antibodies against glycophorin A, an erythrocyte plasma membrane protein.
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