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1. Describe how the concentration of tryptophan influences the transcription of the E. coli trp operon.
Compare this regulation with the lac operon.
2. For the E. coli lac operon, write the partial diploid genotype for a strain that will produce β-galactosidase constitutively (and shows no elevation of levels with induction) and permease by induction.
What manifestation of decreased autonomic nervous system efficiency are seen in elderly individuals?
What is meant by a "stable phenotype" for cells? How can one go about trying to ensure a stable phenotype in vitro?
Explain why it is very important to have a pure culture and not a mixed culture when performing biochemical tests.
diagram the procedure and dilution scheme that you would follow. indicate which plate is likely to be the best plate to count.
is when there are several reproductive castes, not just males and females, but for example when there are three kinds of females that all produce their own young in different ways.
Which process in eukaryotic cells will proceed whether O2 is present or not?
Using Starlings law describing capillary fluid flow, do you think there will likely be a net filtration or absorption or a net flow of zero across the peritubular capillaries surrounding the proximal tubule? explain why.
what are the 4 major macromolecules and list their building block, solubilty in water(hydrophobic/hydrophilic), and specific example of a polymer for each and where it is found
You have 0.05 mL of an undiluted culture at a concentration of 3.6 x 106 CFU/ml. You then add 4.95 mL sterile diluent. what is the dilution, and what is the final concentration of the cells?
how a mutation in a DNA exon will change the eventual mRNA sequence, and how this may or may not change the amino acid sequence.
On a mole per mole basis cells obtain more energy when growing on fatty acids than on glucose. Examine the reactions for degradation of fatty acids and explain why this is true.
leucine has two dissociable protons: one with pKa of 2.3, the other with a pKa of 9.7. Sketch a properly labeled titration curve for leucine titrated with NaOH; indicate where the pH = pK and the region(s) in which buffering occurs. Draw which str..
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