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You are asked to run an SDS-PAGE analysis of samples from an affinity chromatography experiment
performed by your TA. A recombinant protein with a hexahistidine tag was captured in a nickel- agarose bead matrix and eluted off the column with 75mM imidazole. Your TA was hoping to make about 2 mg/mL of the protein. You are given the following samples to prepare and load into an SDS-PAGE gel :
· 10 uL 75 mM Imidazole
· 10 uL pure recombinant protein at 2 mg/mL
· 10 uL recombinant protein in 75 mM imidazole (elution fraction)
1. Describe how you would prepare the three samples to load into the gel.
2. Label the gel lanes with the appropriate contents. The recombinant protein should exhibit one band at 66,000 Daltons
3. Compare the elution lane to the positive control lane. How do you know the recombinant protein present in the elution sample? How pure is the elution sample compared to the control?
4. What assumption can you make about the amount of recombinant protein in the elution sample? What assay can you perform to find the accurate concentration of the elution sample?
What effect might this have on cell lineages during development. Describe the physical changes that would occur in SIM medium if you inoculated an organism that was negative for hydrogen sulfide but positive for indole production and motility.
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Using examples, discuss types of genetic mutations that result in faulty proteins and disease,
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The chloroplast genome of most photosynthetic eukaryotes is roughly five percent the size of the genome of the cyanobacterium from which it is thought to have been derived.
Determine what might be signified if the capillary refill time measures ten seconds in the digits of one had and 2 seconds in the digits of the other hand?
More than a dozen recessive illnesses that are very rare in most of world are fairly common among the Bedouin people who live in the Negev Desert area of Israel.
Create a Dichotomous Key using the following Bacteria. You are only allowed to use the characteristics listed in organizing your Dichotomous Key.
The matrix used in experiment has a fractionation range 20000 to 5x10^6 Daltons. Would you see this matrix purify a plasmid that had a molecular weight.
While using ICD-9-CM codes, there are four main steps that should be followed to make sure that the code is an accurate description of the diagnosis. Using Library and other outside references list and describe the four steps essential to establish t..
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