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You are ready to run your gel but you discover that there is no more running buffer. You need to make 400 ml of 1X TBE containing 0.5 ug/ml ethidium bromide. The stock solution of ethidium bromide is at a concentration of 10 mg/ml. How much of the stock solution should you add to your 400 ml of 1X TBE?
The dye used in the Simmons Citrate Agar is called bromthymol blue. It is green at neutral pH and turns blue as alkalinity increases.
In a population it was reported that the allele frequency for M was 80 percent and for N was 20 percent in the MN blood group. M and N are co-dominant.
Estimate the isoelectric point for the peptide: glu-asp-arg-asn-ser-asp-glu-his-lys-asp Discuss any assumptions made; exact numerical accuracy is not important - approximations may be used.
Compare and contrast a somatic cell with a reproductive cell in terms of cell replication, including processes undergone, resultant descendant cells, and chromosome numbers. Give examples of each.
Wooly-hair is a rare trait found in people of Scandinavian descent in which the hair resembles the wool of a sheep. A woman with wooly hair who has a mother with straight hair moves to an island inhabited by people who are not of Scandinavian desc..
What happens to the post-synaptic cells' membrane potential at an inhibitory synapse?define why the coupling of the import of amino acids.
One in every 685 children born in the chicoutimi area of quebec province has tyrosinemia. It is cause by having two copies of the receissive allele. Under the assumptions of Hardy-Weinberg equilibrium, calculate the value of q required to produce ..
If 1ml of a susension is used to inoculate a plate and 122 colonies are seen on the plate after incubation, how many bacterial cells are present in 1g of meat.
What effect does cell size have on a cell's ability to efficiently carry out it's activities? Give an exam.
You have isolated a new protease that cleaves peptide bonds following Asp and Glu. Based on its inactivation by DIPF, you suspect that it may utilize a mechanism similar to chymotrypsin.
About half of the bacilli and all of the spirilla bacteria are motile, by means of flagella. Very few cocci are flagellated or motile. Why do you think flagella-based swimming motility might be commonly seen in spiral and bacilli bacteria, but ver..
We have three plasmids: the cloning vector, pGEM, the one that result if BamHI had been used to construct the genomic library, and the one that would result if EcoRI had been used to construct the library. What is the expected number of DNA fragme..
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