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Perform the calculations below, showing your working. You need to make 150 mL stock of:25 mM Hepes buffer pH = 7140 mM NaCl12% (w/v) glucose
A) You have a stock of 1 M Hepes buffer pH = 7. How much of this do you need to add?
B) How much NaCl would you weigh out? The molecular weight of NaCl is 58.44.
C) How much glucose do you need to weigh out? The molecular weight of glucose is 180.16.
D) You have harvested and resuspended some cells in 10 mL of this prepared solution, and counted them to find that there are 2.5 x 106cells/mL. You require 5 mL of cells in the same solution, but at 6 x 105cells/mL. How do you dilute the cell stock?
In the mid-1950s, the first study linking cigarette smoking to lung cancer was published. explain how breathing works
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The Km of an enzyme: Vmax:
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you are in charge of developing a new antimicrobial chemical substance that will be used to make food grade cutting
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