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Q1. Sometimes, cDNAs turn out to be "monsters"; that is, fusion of DNA copies of two different mRNAs accidentally inserted adjacent to each other in the same clone. You suspect that a cDNA clone you have isolated from a human heart library is such a monster since the series of the cDNA insert predicts a protein with two structural domains not normally observed in the same protein. How would use the availability of the entire human genome sequence to assess if this cDNA clone is a monster or not?
Q2. Animals obtain energy during a sequence of chemical reaction in which sugar (C6H12O6) and oxygen gas (O2) are reactants. This process produces water (H2O) and carbon dioxide (CO2) as waste products. How might you use radioactive isotope to find out whether the oxygen in CO2 comes from sugar or oxygen gas?
Explain the events that occur when epinephrine binds to a G-protein coupled receptor in the membrane of a cell. Begin with the binding of the epinephrine and end with the activation of adenylyl cyclase.
Assume that, during biological research, it became possible to incorporate fully functional chloroplasts into the basal layer of our epidermal skin cells. Knowing the structure and function of chloroplasts, how will this influence our body.
You now grow Daphnia and Bosmina together. Suppose they remain limited by phytoplankton. What genetic basis of erminette pattern is suggested? How would you test your hypothesis.
List the two kinds of macromolecules that have been partially digested by the time acid chim moves into the intestine. Where did this digestion take place, and what enzymes are involved.
Would you expect the measurements to be equivalent in both preparations? If a variation in size is expected, how will you account for this difference.
On the coding strand above, indicate where you would expect FRP to bind to the DNA. In one sentence, describe why the decision makes sense.
Dialysis patients, who will have blood withdrawn, dialyzed, then replaced, are always weighed while they enter the facility and then weighed carefully before they leave. What is the most probable explanation for this requirement.
If you were asked to create a karyotype from the chromosome spreads you made from HeLa cells in Activity 4.2.1, list materials you would use and explain how you would make the karyotype.
Recognize a group or professional in your area working in the field of biological science. Interview an individual from the organization who works straight in the biological sciences field to develop an appreciation for the type of work, latest resea..
What focal length must a pair of glasses have if they are to correct the vision of a person with a near point of 57.5 cm? The glasses are worn 1.8 cm from the eye.
Relate the functions of lactose, copper, and tyrosine to how these reagents affected phenotype or gene expression of the bacterial transformants. That is, examine how and why the same bacterial sample may look different on each of the various plates.
How can the photosynthesis process be enhanced to fix more CO2 than normal and plant productivity could be increased.
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