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If a person eats contaminated meat from a cow that suffered from mad cow disease, how might that person acquire the disease?
The pUC series plasmid contains LacZ gene with multiple cloning site. Suppose if you insert a piece of DNA into this LacZ gene, how will you tell which one is your recombinant.
Arrange the amino acid substitutions in increasing order of conservatism and amino acids are given using one letter codes to give you some practice in using those.
The parental cross in Drosophila is a mutant male fly (b/b . B/B) with a virgin wild-type female (+/+ . +/+) What are the expected phenotypic ratios for each sex AND the overall population of the F2 generation? There may not be 4 phenotypes for ea..
Explain any possible reasons why different plasmids isolated after the ligation reaction might contain recombinant pieces with different single base changes even though they are all of identical length.
Reiterate this process of progressively adding the cards over a 15-minute period. Specify if you are able to detect an increasingly dim light longer you spent in dark?
Assume you have a pure breeding mutant donkey that has very large teeth and very large ears. Both phenotypes are completely recessive to the normal phenotype.
Explain how to make 50 ml of the following 10X Gel electrophoresis loading buffer, 100 mM EDTA (pH 8.0), 0.1 percent and find mature mRNA.
describe the 5 steps in respiration/metabolism of glucose, address glycolysis, trasition reaction, kreb's cycle, electron transport and chemiosmosis.
Research how a SPECIFIC biotechnology application within the above GENERAL examples (or ones not listed above) is used today. Example: Insulin production in bacteria is an example under the general topic of recombinant organisms.
What is a major difference between mitosis and meiosis I.
After Drosophila DNA has been treated with a restriction enzyme, the fragments are inserted into plasmids and selected as clones in E. coli. With the use of this "shotgun" technique, every DNA sequence of Drosophila in a library can be recovered.
In yeast fermentation, CO2 is produced as a function of time. The production of CO2 by yeast is responsible for the rising of bread. Plot the relative the amount of carbon dioxide produced as a function of time.
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