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1. A student has 35 ml of a crude extract of pig heart. They want to determine how many total I.U.'s of malate dehydrogenase they have in the extract. They take 0.1 ml of the extract and add it to 400 μl of 20 mM MOPS, pH 7.4 (dilution A). After mixing gently, they take 400 μl of dilution A and add it to 800 μl of 20 mM MOPS, pH 7.4 (dilution B). They then run the assay that is listed below: 400 μl 20 mM MOPS, pH 7.4 10 μl 10 mM NADH (in H2O) 10 μl 25 mM oxaloacetate (in H2O) 30 μl of dilution B of their MDH solution, in 20 mM MOPS, pH 7.4 They mix everything gently together and then measure the ΔA340/Δ time. Their measured ΔA340/20 sec. was "-0.065". a) How many total I.U.'s of MDH were in the crude extract? b) The structure of MOPS (3-(N-morpholino) propane sulfonic acid). The molecule is man-made. It has two pKa's, one at the sulfonic acid group (SO3H, pKa around 1.9) and the other at the nitrogen of the morpholino group (pKa 7.2; the N below is shown in its base form). What is the net charge of MOPS at pH 7.4?
If you cross a black fur rabbit and a white fur rabbit, the resulting offspring look gray, when examining closely the fur of gray rabbit consist of both black and white hairs. What type of non-mendelian inheritance is this.
Suppose 'Cell X' contains DNA with 6 different nucleotide and protein consist 32 amino acid. What is the minimum number of nucleotides for encoding one out of these 32 amino acid?
One of the most common complants of pregnant women is the need to urinate often. Explain this, considering the arrangement of the pelvic organ.
Lagniapposis is caused by a microparasite, Lagniappea calcasieuensis, and is transmitted directly by coughing and sneezing. It is highly transmissible and very virulent. Infected hosts become immune after a week if they do not die. Use the equatio..
Name a thyroid hormone that acts to lower blood calcium and a hormone that acts to raise blood calcium. Name two thyroid hormones that affect metabolic rate
Some species of Anopheles mosquito livein brackish water, some in running fresh water, and others instagnant water. What type of reproductive barrier is most obviouslyseparating these different species?
what toxins are found in the blood and how are they neutralised? is an antigen a toxin? ltbrgt
Describe the mechanisms by which a transport vesicle is able to find and fuse with its target membrane. Be sure to include all relevent proteins and processes involved in the recognition, docking and fusion of membranes
Determine which of the following cell-surface receptors leads to cell death and How long does B cell maturation from an HSC to a mature B cell take?
1.you come across a small 4-legged organism approximately 40 cm high weight approximately 6 kg with a feather-like
You ran your samples thorugh a 1% agarose gel. would you expect to get the same results if you ran your samples through 5% agarose gel? Explain your answer.
Examples and classifications of Pisces amphibia mammalian reptilian aves
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