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1. How much 4X SDS-PAGE sample buffer do you need to add to 10 ul of a cell extract to run it out on an polyacrylamide gel?
2. You wish to plate out 5 x 104 lymphocytes in a total of 2 ml for a focus forming assay. You dilute your cells 1:100 with crystal violet and count them with a hemocytometer. The average cell count is 25. What volume of cells would you add to what volume of medium to prepare your assay? Show your work!
3. An antibody to be used for Western blotting needs to be diluted 1:5,000 for optimum results. What volume of the antibody stock solution do you need to add to 100 ml of buffer to make the blotting solution? By the way, what other components should this buffer solution contain?
4. You have 20 ml of a protein sample in a 10% (w/v) suspension of sodium sulfate. How many rounds of dialysis (in a total volume of 1 liter) would be needed to reduce the salt concentration to 1 mg/ml or less?
Compare/Contrast diffusion in the presence/ absence of membranes (carmine powder, gasses vs. ox blood RBC's). What do they mean by this ?
1.How are interstitial fluid and lymph similar, and how do they differ? 2.What are the roles of the thymus and the lymph nodes in immunity? 3.Describe the functions of the spleen and tonsils
which of the following has the greatest effect on the rate of chemical cycling in an ecosystem?
Which of the following tumors can be surgically resected with no residual consequences?
A young woman distance runner has recently not been performing well and has complained of feeling lethargic and sluggish. You perform a graded exercise test to VO2max including blood samples to attempt to determine possible cause.
Discuss similarities and differences of homeostatic mechanisms for thermoregulation in ectothermic and endothermic animals.
what are some of the methods that scientists have used to study the components, processes, and outcomes of cell division?
Explain how the cell anatomy of the non-ciliated simple columnar epithelial lining of the small intestines cpmtributes to the overall function of the small intestines.
permeability is the condition of being capable of having materials flow into and out of a membrane. the permeability of
Draft Research Questions
a. discuss three instances in which a microbiologist must utilize a pure culture.b. describe four of the instruments
What is the size of LH and the size of progesterone receptors
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