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An oligomeric protein has a molecular weight of 367000. A 460 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCl. The product of the complete hydrolysis contained 2.2 mg of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the ?-amino groups of other amino acids could be found. How many polypeptide chains are in the protein?
Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end "sticky" and why is this especially useful for inserting DNA fragments into plasmid DNA?
Some plant breeders are concerned about the resistance of asexually cultivated plants, such as seedless bananas, to new strains of disease-causing bacteria, viruses, or fungi.
Assume you leave a culture inthe 37 C incubator with out tryptophan for week instead of overnight like you planed. to your surprise culture has shown some visible growth so you reinoculate from that flask into a new flask
In squash, disc shaped fruit is dependent on dominant gene D and elongated fruit on recessive allele d. Yellow color is controlled by dominant gene R and green color by recessive allele r. Draw a cross between the homozygous squash fruits and summ..
Key factors which influence respiratory rate and depth are such as talking or coughing, (such as, sometimes we try to breath at a several pace to relax)
A triploid cell containes sets of three homologous chromosome is a cell of used chromosome per cell is triploid this cell would be expected to have which of the.
Steve is in the midst of his Microbe experiment. He is increasing mold on a piece of peach. After five days in the baggie, the mold covers 88 square millimeters of the peach surface.
Plasmid size of pGLO is 5371 bp and the restriction sites for Pst I are 2106 and 3181. Compute to predict the sizes of fragments when gPLO is digested with PstI.
Compare and contrast the mechanisms of transcription initiation in prokaryotes and eukaryotes. Suppose you were to count one coliform colony produced from a 10 ml water sample.
Three groups of cyclists shown above consumed 3-different types of diets: high-carbohydrate, a diet mixed in carbohydrates, or a diet higher in protein and fat.
If two parents are heterozygous carriers of CF, show a pedigree with four children showing the incidence of carriers and afflicted children. If each unafflicted child (carrier or not) has a 1 out of 30 chance of mating with another carrier of CF, ..
Who is the responsible Staff member or Physician of these: Fact sheet Admission consent Nurse assessment Physician orders Admin History and physical Laboratory Test results Operative notes Discharge Instructions Discharge Summary I have them answe..
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