How many micrograms of dna will you need for transposition

Assignment Help Biology
Reference no: EM13112865

What if you were using a plasmid that was 10 kb in size. You still want to use .05 pmoles of your target DNA. How many micrograms of DNA will you need for the transposition reaction?

Reference no: EM13112865

Questions Cloud

Compare the processes of body axis : Compare the processes of body axis determination in Drosophila to that in vertebrates. How is this process in insects and vertebrates similar and how is it different?
Explain how would you compare advantages and disadvantage : explain how would you compare the advantages and disadvantages of using exports, joint ventures and wholly owned subsidiaries as ways of expanding international sales?
Determine the mass percentage of cadmium sulfide : If the sample reacted completely and produced 1931 mL of H2S hydrogen sulfide at 29.82 °C and 759.7 mm Hg, determine the mass percentage of Cadmium sulfide in the mixture?
Fundamental flaw in procedure : Tom Smith wants to test the hypothesis that adult men have a mean weight that is greater than 150 pounds. Tom surveys the adult male members of his family and obtains 40 sample values
How many micrograms of dna will you need for transposition : What if you were using a plasmid that was 10 kb in size. You still want to use .05 pmoles of your target DNA. How many micrograms of DNA will you need for the transposition reaction?
Illustrate what steps would you recommend your manager take : Illustrate what steps would you recommend your manager take to implement the planning technique you chose? Make sure you support your work.
How much potassium nitrate remains in solution : A mixture of twenty five grams of potassium nitrate(KNO3) and two grams of CuSO4*5H20 with 11.5 grams of water at 100 degree celcius temperature, 15 g ramsof water are mixed.
Determine the pressure inside a container of a gas : Determine the pressure inside a container of a gas connected to an open-end manometer filled with CCl4, has the density = 1.594 g/cm3,
Find mean number of accidents per twenty four hour period : If the probability of no accidents during a 24 hour period is 0.1353, what is the mean number of accidents per 24 hour period?

Reviews

Write a Review

Biology Questions & Answers

  Determine fraction of the molecules

If C-1 of glucose is radioactively labeled & then enters glycolysis, which carbon of G-3-P will be labeled? Determine what fraction of the molecules will carry radioactive label?

  Different methods of transporting molecules into cells

What are the the five different methods of transporting molecules into cells. Describe each of the methods.

  Mechanisms of action of antiviral drugs to influenza

What are the targets and mechanisms of action of antiviral drugs to influenza and why are there so few antivirals to influenza available?

  Question about different gametes

Discuss how many different gametes are formed in a test cross when the cross examines one gene, two genes and three genes?

  Determine length of the restriction fragment

Two NotI restriction enzymes cleave DNA on opposite sides of the Dbm gene in a species of yeast. A molecular probe for Dbm detects a DNA restriction fragment of 8.5 kb in organisms that are wild type at Dbm.

  Calculate frequency of the two alleles

The MN blood group is determined through a single gene with two alleles M and N. A Navajo population in New Mexico was tested for this blood group.

  Forensic biology

Assume you found your sibling's brother-in-law has had a sample submitted to the lab. This is a person you have never met, but that you know has been in trouble with the law.

  Biology question

I placed some diastase powder in some starch solution in a test- tube. By taking samples from the tube and testing them with iodine,

  Explain facilitated diffusion of glucose into muscle cell

The compound ouabain is a specific inhibitor of the active transport of Na+ out of the cell. For each of the following precesses in your body, would you expect the process to be sensitive to inhibition by ouabain?

  What you have learned about antigen processing

An attenuated active ("live") viral preparation that has low virulence but can still invade and replicate in host cells.

  Find disadvantages of taking low dose aspirin

It has been proposed that daily low dose aspirin can be used for primary prevention of cardiovascular disease. However there are risks associated with taking aspirin. Outline the possible advantages and disadvantages of taking low dose aspirin, by..

  Compute the keq for the hydrolysis

The delta G for hydrolysis of the amide group from glutamine is -14.2 kJ/mol at 25C. Compute the keq' for this hydrolysis.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd