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The microtubules of an axon are thought to be nucleated at the centrosome, then severed from their nucleation site and moved into the axon. It was noted in the text that cytoplasmic dynein is responsible for the retrograde movement of organelles in axons, yet this same motor is thought to mediate the anterograde movement of microtubules in these same cellular processes. How is it possible that the same minus end-directed motor can be involved in both anterograde and retrograde movements?
Normal cells need to be attached to the extracellular matrix to survive and proliferate. Explain this in terms of outside-in integrin signaling.
A double-stranded fragment of viral DNA, one of whose strands is shown below, encodes two polypeptides called vir-1 and vir-2. Adding this double stranded DNA fragment to an in vitro transcription and translation system yields
A population of fifty-thousand around a nuclear facility receives an average wholebody dose of 0.5 rad as a result of an accidental release of radionuclides.
The very simple genotype-phenotype relationship of dominance, codominance and recessiveness has been complicated by issues of penetrance and epistatsis.
Some restriction enzymes produce fragments that have single stranded ‘sticky ends', while other enzymes do not leave single stranded ends.
The unfenced control plot had not changed significantly in species composition. By means of the concepts discussed in the chapter, what do you think happened.
suppose a bird that eats grasshoppers moves into the prairie. How will this affect natural selection of the grasshoppers? How might this change in a drought year.
You are studying 2 wetlands in eastern Ohio - one that has become highly invaded by purple loosestrife. These wetlands typically contain over one hundred species.
After the microarray slides were hybridized with labeled cDNAs derived from these 5 populations of RNAs, the data were analyzed by several different comparisons.
In the dark reaction, plant cells can fix CO2 into glycealdehyde-3-phosphate (G3P) in the stroma of chloroplasts, then into glucose or sucrose (sugar) in cytoplasm.
1 µl containing 1 ng of supercoiled Bluescript plasmid DNA was mixed with 50 µl of electrocompetent cells. The cells were electroporated and 500 µl of SOC media was immediately added and the cells were incubated at 37 C for 1 hour to allow them to re..
You wish to fuse a cDNA sequence to the end of two tandem"HA" antibody epitope tags within the MCS-HAHA region of the vector given below.
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