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1. How does the Histidine "tag" allow us to purify the GFP?
2. How is GFP eluted from the nickel agarose beads? Can you think of an alternative way of doing this?
3. Why is a hexa-histidine tag used, rather than another length of histidines (e.g. deca-histidine)?
4. Can you think of a downstream application where it would be undesirable for a purified protein to have an affinity tag attached? How could you come up with a construct where the tag is removable or remove an existing tag?
5. How does lysozyme aid in bacterial lysis?
6. What is sonication? Why do we sonicate our bacterial cells?
7. Why are DNase and MgCl2 added to the lysed bacteria?
8. We will examine aliquots of pre-induction and post-induction bacterial culture by SDS-PAGE. Considering that these aliquots will contain differing amounts of bacteria, how can we ensure that we apply suitable and comparable levels of protein to the gel?
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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