Reference no: EM13922832
1. How do retroviruses such as HIV differ from LTR-type retrotransposons?
2. Why is it important for transposons to regulate the frequency at which they transpose?
3. Given that modern-day strains of corn, widely grown in Illinois, contain thousands of copies of Ds elements, why are these lines so genetically stable?
4. In your studies of IS elements of E. coli, you have identified a unique element. This IS, called IS42, is non-autonomous and requires IS5 in the same cell in order to transpose. You observe that insertions of IS42 into the lacZ gene of the lac operon are mutagenic on lacZ, but do not exert polarity on the downstream genes, lacY and lacA. You sequence the transposon and find that:
A. It produces 7 bp target-site duplications.
B. It is 119 nucleotides in length including its 12 bp IR elements.
C. It does not contain any transcriptional termination signals or stop codons in any reading register in either DNA strand..
How is it that this IS element can transpose?
5. Describe three roles of the poly dA sequence located at the 3' ends of non-LTR, poly-A containing retrotransposons.
6. In replicative transposition in which the transposon moves from one replicon to another, how is the cointegrate intermediate (the fused replicons) that is produced during transposition converted back to the two replicons?
7. You know the mechanism by which a transposon can move from one site in a DNA molecule to another site in the same molecule or to a site on a different DNA molecule IN THE CELL. But how can a bacterial transposon move from one cell to another?
8. Target site duplications are seen at the sites of transpositon of bacterial transposons and both LTR and non-LTR type eukaryotic retrotransposons. How do these duplications arise and what do they suggest in terms of mechanisms of transpositon?
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Involving national public accounting firms
: Litigation, Censures, and Fines [Pin It] Assignment 2: Litigation, Censures, and Fines Due Week 7 and worth 280 points Research the Internet for recent litigation, censures, and fines involving national public accounting firms. Examples of litigat..
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Subsidiary had common stock
: McClellan, Inc. acquires all of the outstanding common stock of Cranston Enterprises for $150,000 cash. On the acquisition date, the subsidiary had Common Stock of $30,000 and Retained Earnings of $30,000.
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Expected sales mix is three of the small drives
: The expected sales mix is three of the small drives (1GB) for every one large flash drive (2GB). Given this sales mix, how many of each type of flash drive will flash Co. need to sell to reach its target monthly profit of $ 260,000
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Why should there be a delay in developing new organisation
: ‘If structure does follow strategy, why should there be a delay in developing the new organisation needed to meet the administrative demands of the new strategy?' (Alfred Chandler) How would you answer this question
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How hiv differ from ltr-type retrotransposons
: 1. How do retroviruses such as HIV differ from LTR-type retrotransposons? 2. Why is it important for transposons to regulate the frequency at which they transpose?
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Differences between financial and managerial accounting
: What are the differences between a direct cost and an indirect cost? Which is the more difficult cost to track? Why? How do indirect costs affect the cost of a product? Should indirect costs be included in product cost? Why or why not?
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Identify the key information related to current activities
: Identify TWO examples of conflict that could be expected to result fromstaff adopting a strict adherence to only their current perceived primaryroles. One example should evidence negative conflict, while the otherexample should evidence positive c..
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What happens when employees and manager are brought together
: ‘All any company has to do to explore its own potential to become a more innovatory organisation is to see what happens when employees and managers are brought together and given a significant problem to tackle.'
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Determine the regression coefficients b0 and b1
: Construct a scatter plot. Use the least-squares method to determine the regression coefficients b0 and b1
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