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As you complete the lab, answer the questions below, when completed save with your name and then attach to the Lab 6 drop box. Experiment 1: Diffusion through a liquid 1. A concentration gradient affects the direction that solutes diffusion. Describe how a molecules move with respect to the concentration. 2. How does the size of a solute affect the rate of diffusion? Consider the size and shape of a molecule in your response. 3. Does polarity affect the rate of diffusion? Explain your answer using scientific principles. 4. After completing experiment 1, fill out the tables below and answer the questions following: Table 1: Diffusion Through Liquids Corn Syrup in mm Time (sec) Blue Dye mm Red Dye mm 10 20 30 40 50 60 70 80 90 100 110 120 Table 2: Diffusion Rate of Dyes in Corn Syrup Dye Name Molecular Weight Total Distance Traveled (mm) Speed of Diffusion (mm/hr)* Blue Dye Red Dye 1. Record your hypothesis from Step 3 here. Be sure to validate your predictions with scientific reasoning. 2. Which dye diffused the fastest? 3. Does the rate of diffusion correspond with the molecular weight of the dye? 4. Does the rate of diffusion change over time? Why or why not? 5. Make your own line graph using your data and insert as .jpg in your report here. 6. Explain the role diffusion plays in this process of getting oxygen to your cells of the human body. Experiment 2: Concentration gradients and membrane permeability Table 3: Indicator Reagent Data Starch + Conrol (color) Starch Negative Control (color) Glucose + Control (color) Glucose Negative Control (color) IKI Solution N/A N/A Glucose Test Strip N/A N/A Table 4: Glucose Diffusion Dialysis Bag after 60 minutes Beaker water after 60 minutes IKI Solution Glucose Test Strip Experiment 2: Do experiment on Lab 6, Diffusion Questions 1. Why is it necessary to have positive and negative controls in this experiment? 2. Include a picture of you and your experimental set-up here: 3. Which substance crossed the dialysis membrane? What evidence from your results proves this? 4. What molecules remained inside of the dialysis bag? 5. Of the substances that diffused through the bag, did all of the molecules diffuse out? Explain. 6. Is the bag hypotonic with regards to the IKI solution, or the beaker? What about the starch solution?
7. What results would you expect if the experiment started with glucose and Lugol's Solution inside of the bag, and starch and water in the beaker? Why? 8. Draw a diagram of this set up. Use arrows to depict the movement of each substance in the dialysis bag and the beaker.
9. What type of membrane does the dialysis tubing represent? Give an example of this type of membrane that can be found inside the body. 10. How does the glucose concentration affect diffusion rate?
Assume you examine the stability of your favorite protein kinase and find that half of the protein is degraded every 10 minutes.
The Voges Proskauer (VP) test detects a specific product of a mixed acid fermentation, which distinguishes the genus Escherichia from Klebsiella and Enterobacter. What is the biochemical basis for the VP test?
You are a manufacturer of boxes used for take out Chinese food. A supplier comes to you and asks you for help in solving his problems. When people order food in the family size.
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If you analyze the diameter of the field of view for the 4X objective lens of a compound microscope with 10X oculars as 4.5 mm, what is the diameter of the field of view for the 100X objective lens?
red hair autosomal recessive is found in approximately 4 percent of the people in norway. if we assume that the
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The membranous vesicle of a submitochondrial particle after removal of the F1 spheres would be able to (1) oxidize NADH; (2) produce H2O from O2; (3) generate a proton gradient; (4) phosphorylate ADP.
1.how is the nervous system of the flatworm different from that of a human?2.describe the nonspecific defenses that
One subunit of tropomyosin is homologous to calmodulin. What would you predict the role of that protein would be?
Rank the given fatty acids from highest melting point to lowest melting point:
What frequency of homozygous recessive genotypes is predicted by Hardy-Weinburg and In 500 people are born with at least one copy of a potentially deadly allele that cause hyper - choleseterolemia
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