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1. Normal cells greatly slow their rates of cell division after filling a culture dish with a layer one cell deep (a monolayer). If a circular group of cells is removed from the middle of the filled culture dish, the cells on the edges of the "wound" will begin to divide, fill the space and then stop dividing again. How does the behavior of transformed cells differ? 2. You look at two slides in the microscope. The first contains cells displaying nuclear irregularity and a scant and disorganized cytoskeleton. The second contains cells with a normal chromosomal number and a well-organized network of microtubules, microfilaments and intermediate filaments. Which, if any, contains cancer cells? Explain your selection. 3. You grow some cells in culture and measure their growth rate. You then add serum with growth factors in it to the culture medium and once again measure the growth rate. The growth rate increases only slightly. What kind of cells are these likely to be and why do you think so?
How does the intensity of simple diffusion vary in relation to the concentration gradient of the moved substance and how does the intensity of facilitated diffusion vary in relation to the concentration of the moved substance
Mary is in an automobile accident and injures her spinal cord. She has lost feeling in her right hand and her doctor tells her it is the result of swelling compressing a portion of her spinal cord. Which part of her cord is possible to be compressed.
Why is co-transfection of two promoter-reporter plasmids always required when conducting these kinds of experiments? Briefly, describe how this is done.
Both proto-oncogenes and tumor suppressor genes can interact with transcription factors involved cell division. Determine the fundamental difference in how the two function?
The time-of-entry mapping method was used to determine the first five markers transferred from each Hfr during a synchronized conjugation experiment. The markers and their transfer order form each Hfr are shown below.
In corn plants, a dominant allele I inhibits kernel color, while the recessive allele i permits color when homozygous. A second dominant allele P produces purple kernel color, while the homozygous recessive genotype pp causes red kernels.
What percent of the population are homozygous dominant. What do you assume would be the effect on the switch of reversing orientation of one of the hix sites.
What is the function of introns? Are they just evolutionary baggage?
The information to the below were collected for the bovine carbonic anhydrase-catalyzed reaction described by, Use these values to determine the value of KM,
the circulatory system of an amphibian is divided into two separate loops. the pulmonary circulation carries
In a generalized transduction experiment, phages are collected from an E. coli donor strain of genotype cys+ leu+ thr+ and used to transduce a recipient of genotype cys- leu- thr-.
Explain briefly the division of the cerebrum into hemispheres and lobes. Determine what are the primary functions of each lobe?
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