How does one achieve limited protease digestion

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Reference no: EM133290654

Assignment:

Short Answer the following questions:

1. Why is proofreading by the 3' ro 5' exonuclease activity of DNA polymerase I during DNA synthesis very important?

2. How does one achieve limited protease digestion?

3. Why are two different primers required for the PCR reaction?

Reference no: EM133290654

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