Reference no: EM133309015

Question 1) The myeloproliferative neoplasms are a group of blood cancers which can lead to excess blood cell production in the bone marrow and often in the peripheral blood. Many blood and genetics test are applied in order to describe a MPN (myeloproliferative neoplasms).
The chronic myeloid leukemia is caused by the mutation on the gene BCR-ABL1. Testing peripheral blood by fluorescence in situ hybridization (FISH) for the BCR-ABL1 fusion gene is the first initial test to detect this disease.
Do you think the PCR technique can be employed to identify this mutation? How?

Question 2) For the early identification and targeted treatment of myeloproliferative neoplasms which is a kind of chronic haematological tumour that is accompanied by leukaemia or bone marrow failure, a collaborative team from China recently developed a sensitive, multiplexed, quantitative detection PCR approach. This Digital polymerase chain reaction approach is a high-sensitivity, absolute quantitative, and high-tolerance nucleic acid detection technology, that is crucial in several application fields, including the detection of rare mutations, copy number variation, liquid biopsies, single-cell analyses, the detection of genetically modified organisms, viral load testing, and microbiological quantitative analysis. Nanoparticles and PCR additives were used to get suited for nanoliter-scale amplification and the conventional loop-mediated isothermal amplification (LAMP) was modified. Cerebrospinal fluid and aqueous humour are two examples of micro samples that are particularly well suited for highly sensitive detection by dPCR. Chip-based dPCR (cdPCR) method with high isolation stability, good temperature uniformity, and quick detection speed was created in the digital PCR study and development.

Question 3) From this paragraph, this question needs to be answered.

Regarding dPCR analysis, apart from cerebrospinal fluid and aqueous humour, is it possible to use blood sample or any other sample? I'm asking because obtaining cerebrospinal fluid is very risky because any error may lead to paralysis. And is the technique given in 2nd paragraph is cheap or expensive?