Solution-How do you dilute the cell stock

How do you dilute the cell stock

Reference no: EM132751529

Perform the calculations below, showing your working. You need to make 150 mL stock of:25 mM Hepes buffer pH = 7140 mM NaCl12% (w/v) glucose

A) You have a stock of 1 M Hepes buffer pH = 7. How much of this do you need to add?

B) How much NaCl would you weigh out? The molecular weight of NaCl is 58.44.

C) How much glucose do you need to weigh out? The molecular weight of glucose is 180.16.

D) You have harvested and resuspended some cells in 10 mL of this prepared solution, and counted them to find that there are 2.5 x 106cells/mL. You require 5 mL of cells in the same solution, but at 6 x 105cells/mL. How do you dilute the cell stock?

Reference no: EM132751529

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How do you dilute the cell stock : Perform the calculations below, showing your working. You need to make 150 mL stock of:25 mM Hepes buffer pH = 7140 mM NaCl12% (w/v) glucose

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How do you dilute the cell stock

Reference no: EM132751529

Reference no: EM132751529

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