How do you design experiment to test that adenylyl cyclase

Reference no: EM133380328

Questions:

1. Understanding Background information: In the canonical pathways (pathways that have been discovered in other organisms-not in spiny lobster), Dopamine (DA) modulates potassium channel (IA) in neurons by through several highly conserved dopamine receptors (DR) that belong to the G-protein-coupled receptor (GPCR) superfamily. Type 1 DA receptors (D1Rs) couple with Gs to increase adenylyl cyclase (AC) activity and type 2 DA receptors (D2Rs) couple with Gi/o to decrease AC activity, thereby increasing and decreasing cAMP levels, respectively.

2. Constructing a hypothesis for your own your research: You are working in a molecular biology laboratory to study DA signal transduction pathway in spiny lobster. You already discovered that DA alters rhythmic behaviors (food digestion) of spiny lobsters by modulating potassium channel (IA). You have also identified the presence of D1Rs in a specific type of neuron. What is your hypothesis regarding to how DA modulate potassium channel (IA)?

3. Experiment design 1: How do you design experiment to test that adenylyl cyclase (AC) is involved in DA modulation of IA. Forskolin, which is a chemical that can mimic the effect of DA is available in the lab. 4. Experiment design 2: How do you design experiment to test that protein

Reference no: EM133380328

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