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12. How do restriction enzymes and ligase allow us to clone fragments of DNA?13. How are cloning vectors then put into bacteria cells?14. How do we know if the bacteria cell has the vector? How do we know if the vector contains the insert?15. How is a cloning vector different from an expression vector?16. How does a Eukaryotic gene have to be modified before being expressed by a bacteria cell?17. Define: transformation, X-gal, Ti plasmid,18. How is PCR similar to cloning? What are the advantages to PCR over cloning?20. What are primers? How do they specify which segment of DNA is going to be amplified?21. If we don’t know the exact sequence of the gene, then describe 2 ways in which we can still use PCR to amplify that gene?
dna and rna are similar yet distinct components of the cell. describe three differences between rna and dna with
What is the probability that an offspring will inherit cystic fibrosis, an autosomal recessive disorder, if both parents are carriers?
Compare DNA to a cookbook. The book is kept in a library and cannot be checked out (removed). Start with the letters and words in the cookbook compared with the bases and codons in DNA
Among Cucasians, neither straight (HsHs) nor curly hair (HeHe) is dominant (i.e., this trait shows incomplete dominance). When a curly haired person reproduces with a straight haired person,
Compare the effects of the destruction of the spinal nerves dorsal root ganglion and the destruction of the ventral roots.
Draw a dichotomous key to use in the identification of your unknown. Our strains will be grown on solid media, so cell arrangement will probably not be diagnostic.Notice that the objective is to use the tests .
Two normal parents produce a child with PKU, a recessive genetic disease? Determine the probability that a sperm from the father will contain the PKU allele?
1000 molecules are bouncing at 300K in two wells separated by a free energy gap DG = 6·10-21 J. Compute how many particles on average are in each well.
The sexually transmittable disease gonorrhea has become increasingly resistant to treatment with antibiotics. What is the most likely scientific explanation?
Tryin to determine the independent and dependent variables for a biology question and the control group.
draw a glycerol and explain how a triglyceride is formed from this molecule and fatty acids.
define a well-controlled experiment to determine if the rise in internal free calcium observed in the cytoplasm of unfertilized sea urchin eggs in the presence of A23187 is due to in flux of calcium from seawater. Include experimental treatments, ..
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