Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following.
a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line.
b) Describe how you would measure specific receptor binding of GH to your stable cell line.
c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls.
d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.
In thick-billed murres, fledglings plunge into the ocean from their hundreds-meters high nesting cliffs. List the reasons for the lack of developmental and genetic variation in allometric proportions of chicks in this species.
"Make a chart or table in Microsoft Word to compare and contrast cellular and humoral immunity" including the following factors: Development
what is a plate count and why would it be used instead of direct microscopic counts or turbidity measurements?
Explain how a molecular biologist could determine whether acut-site was in the middle of an antibiotic- resistance gene. Explain how restriction enzyme digestion results would change if aforeign gene was inserted into a plasmid.
Charlie is badly burned in a fireworks accident on the Fourth of July. When he reaches the emergency room, the examining physician determines the severity of the incident as a third-degree burn. What are the major concerns with third-degree burns
what will be all the constituents of the medium used to select exconjugants that have aquired the ala+ allele?
Dinitrophenol (DNP) is an "uncoupler" that causes membranes to become permeable to H+. Explain what would happen if DNP was added to plants that were carrying out photosynthesis. Would the light and dark reactions still proceed?
how the blood is circulated with the help of heart
Moniliasis is one of the most common opportunistic diseases in AIDS. What is the etiological agent of moniliasis and what is the other name of the disease?
Why can't microbes that naturally clean up the environment continue to do so as in the past? How have humans made their task harder? Easier?
Discuss the role of PINK1 and Parkin with regard to Parkinson's Disease.
An individual with a disease demonstrates a set phenotypic presentation, Another individual with the same phenotypic presentation is discovered. When we sequence their genome, we find that there are two distinct DNA sequences involved, both differ..
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd