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1. Which of the following enzymes is directly involved in base excision repair mechanism of mutations?GlycosylasesCyclic Adenosine MonophosphateSerines KinasesHelicasesAlkytransferases2. Shown below is a list of types of repair systems or repair molecules (a-g) and types of DNA damage or mutations (1-6). On the blank line following each type of damage, write the letter(s) of all repair systems that can repair (or play a role in the repair of) that particular type of damage.a) Photolyaseb) Base-excision repairc) SOS repaird) Alkyltransferasese) Mismatch repairf) Homologous recombinationg) Nucleotide-excision repair1) Alkylation of G residues __________2) Trinucleotide repeat expansion__________3) Depurination __________4) Pyrimidine dimers __________5) Deamination __________6) Double-stranded breaks __________ 3. Determine which of the following repair pathways do not involve the use of a homology-dependent repair system?Base excision repairPhoto reactivation repairTranscription coupled repairNucleotide excision repairMismatch repair4. What repair system exists in cells to repair damage of a single base via homology dependent repair? Describe the mechanism of this repair system.5. Put the following events in order as they occur in recombination according to the "double-stranded-break model."a. Cleavage of Holliday structuresb. Formation of loop in uncut duplexc. DNA repair synthesis of second strandd. Strand invasione. Double-strand break of one chromatidf. Formation of Holliday structuresg. DNA repair synthesis of one strand
What combination(s) of chromosomes are possible in haploid cells following both divisions of meiosis? (Hint: remember independent assortment). A diploid cell contains three pairs of homologous chromosomes designated C1 and C2, M1 and M2, and S1 and S..
What is hydrogenase? How can this enzyme be used as part of a strategy to "improve" a Rhizobial strain used to inoculate legumes? Describe logic and provide the appropriate diagram.
Compute the change in energy of an atom that emits a photon of wavelength 2.21 meters. (Planck's constant is 6.626 x 10-34 joule seconds; the speed of light is 2.998 x 108 m/s)
Ethanol is supplied in a 95 percent solution. You need 50mL of 70 percent Ethanol for your experiment. How would you make this solution?
find the sequence of amino acids coded for by this mRNA.
Determine what is the most common mechanism for repairing damage to DNA? Individuals with xeroderma pigmentosum inherit a faulty DNA repair mechanism.
Provide many examples of soluble and insoluble dietary fibers. List food sources of these fibers. Contrast the physical characteristics and features of these two types of fiber and their effects on gastrointestinal tract function.
Determine which of these would synthesize the enzymes for Histidine synthesis
Each lymphocyte in circulation changes over the life of the cell and develops certaincharacteristics, particularly after an encounter with an antigen. Determine which organic macromolecules are most antigenic?
Assume y ou are analyzing a dominant allele in fruit flies that mutates to a recessive allele at the relatively high rate of 10 -6 per generation.
If you transform bacteria with the plasmid library made in part b above, divide the transformatnts into 20 aliquots and find one has the insert of interest, now how many clones do you need to screen.
A drug has a molec. weight of 200. Determine how many grams would you use to make 100 ml of a solution that is isotonic? Suppose that the diluent is distilled water and that the drug does not ionize in water.
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