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Problem- You are interested in how tRNAs might be inhibited in vivo to the addition of high concentrations of the metabolic cofactor FMN which normally should not be a problem for a cell. But you notice that relatively high concentrations of FMN, specifically above 100 ?M and at elevated temperatures, appear to inhibit E. coli growth and you have traced it to some sort of interactions between FMN and e coli glutamine tRNA. Thus you decided to do an in vitro SELEX experiment, starting with the wild-type E. coli tRNA Gln( CUG) sequence, to discover strong FMN aptamers at 48 ° C. Describe in detail how you would carry out these experiments and comment on whether the results that you get might have any beating on your observations of growth inhibition in vivo or not.
I need help for above question. Show each steps for the above problem
A carboxylic acid can exist in two conformations E and Z that are different by the dihedral angle around the C-O bond. Recent theoretical calculations indicate that the Z conformer of formic acid
A weather balloon is filled to 125 L when the atmosphereic pressure is 748 mm Hg and the air temperature is 28 degrees celcius. It rises to where the atmospheric pressure is 787mm Hg and the temperature
Problem- Compute the total number of possible decapeptides that could be synthesized from the 20 standard amino acids. If you were to spend five minutes writing out the molecular structure of each possible decapeptide how long would the task take
find the reaction conditions eg tempreatures,conditions, Pressuresetc for the process. I also would appreciate any information regarding the disposal of the waste products or recycling of them.
The initial volume of the balloon is 5.00 L, and the pressure is 760 mmHg. The balloon ascends to an altitude of 20 km, where the pressure is 76.0 mmHg and the temperature is -50 degrees celsius. What is the new volume,V2 , of the balloon in liter..
Question- Note the electronic structure of Cadmium (II) and indicate the group (type A, borderline, or type b) in which it should be placed. Predict the principal inorganic forms of the element in fresh and sea water under both oxidizing and reduc..
The vapor above a mixture of pentane and hexane at room temperature contains 35.5 pentane by mass. What is the mass percent composition of the solution
the average ph of precipitation in rural areas is 6.80. assuming that the phnbspis controlled by the carbonate system
a bacterial culture isolated from sewage produced 35.0 ml of methane ch4 at 31degc and 753 mmhg. what is the volume of
imagine now that one tried to repeat the same measurement but between the time that the 480 cm3 of water was measured
Calculate the percent ionization of hydrazoic acid (HN3, Ka = 1.9x10^-5) in solutinos of the following concentrations. A) 0.400 M
A solution of 75.0g of benzene and 75.0g of toluene has a total vapor pressure of 80.9mmHg at 303K. Another solution of 100.0g of benzene and 50.0g toluene has a total vapor pressure of 93.9mmHg at this temperature.
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