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Several cell types have been proposed to create niches for Haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem cell factor (SCF; also known as KITL) is a key niche component that maintains HSCs. Here, using Scfgfp knock-in mice, we found that Scf was primarily expressed by perivascular cells throughout the bone marrow. HSC frequency and function were not affected when Scf was conditionally deleted from haematopoietic cells, osteoblasts, nestin-cre- or nestin-creER-expressing cells. However, HSCs were depleted from bone marrow when Scf was deleted from endothelial cells or leptin receptor (Lepr)-expressing perivascular stromal cells. Most HSCs were lost when Scf was deleted from both endothelial and Lepr-expressing perivascular cells. Thus, HSCs reside in a perivascular niche in which multiple cell types express factors that promote HSC maintenance.
Please provide 4 peer-reviewed articles which are specifically related to strategic alternatives in health care organizations.
Illustrate out the structuration theory, and why is this theoretical perspective unique in comparison to the other theoretical perspectives you have covered in this course?
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John was shocked when his doctor informed him that his condition was caused by extensive damage to his hammer, anvil also stirrup. What condition is John suffering from.
Using behavioral/social learning theorists (Skinner, Bandura, Rotter), a psychoanalyst told me I needed 3 years therapy to explore Oral/Anal fixation.
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You're a leader in global organization and have been asked to develop a presentation to the Board of Directors regarding the importance of diversity-conscious organization.
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