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You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following.
a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line.
b) Describe how you would measure specific receptor binding of GH to your stable cell line.
c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls.
d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.
explain how a decrease in ph and oxygen levels in the blood and interstitial fluid affects the heart blood vessels
The key functions of digestive system are the ingestion of food, ______ of food through the digestive system, ______ by ______ and ______ digestion, ______ and ______.
What is one example of Agesim that you have witnessed in healthcare? How do you think agesim affects older adults? What do you think nurses can do to stop agesim in healthcare settings?
A father of a certain family belongs to blood group AB, and the mother to blood group O. They have four children, one belonging to blood group AB, one to group A, one to B and one to O.
Which animals have four-chambered hearts? The barrier to invasion by microbes involves?. Which are divided into two groups: T cells and B cells? Macrophages, lymphocytes, complete cells, or platelets
Select one:a. It is the larger of the two circulatory systems.b. It is a low-pressure system that allows for improved gas exchange.c. The system functions with an increased arterial pressure to circulate through the distal parts of the body.d. It con..
Based on your reading of the GCU Introduction and the textbooks, what is the main difference between the modern Western worldview and the postmodern worldview? Which parts, if any, do you identify with? Explain. Cite references from your reading t..
A microbiologist must culture a patient 's feces for in testinal (enteric) pathogens. Which of the following meida would he likely use. What is the purpose of resazurin in reducing media.
Give a probable explanation for the inheritance of the melanotic mutation. A 19 year old model for Glamour magazine tripped over an extension cord causing injury to her epidermis. She is afraid injury will leave a scar. Based on your information of r..
What spiritual issues surrounding a disaster can arise for individuals, communities, and health care providers? Explain your answer in the context of a natural or manmade disaster. How can a community health nurse assist in the spiritual care of t..
The table presents the rates of reaction at specific substrate concentrations for an enzyme that displays classical Michaelis-Menten kinetics. Two sets of inhibitor data are also included.
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