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Gfp isolation and western blot analysis

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Reference no: EM132940433

GFP Isolation and Western Blot Analysis:

Explain the presence of the western blot under the "negative" sample.

  1. If you eluted the GFP protein with a high salt buffer from the column, what would you see in that fraction three and why?
  2. If you mix the GFP with a high salt buffer and run through the hydrophobic column, what would you see in that fraction one and why?
  3. If you fail to detect GFP in fraction #3, what could be the reason? Assume you successfully isolated GFP in fraction #3. Provide two possible causes.
  4. Why did we add lysozyme and place the tube at -800C?

Reference no: EM132940433

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