Reference no: EM132669979

Genetic Engineering Discussion Questions:

1. Ampicillin (AMP) resistance is a selectable marker located on the pGLO plasmid.

a. What is the purpose of the selectable marker?

b. What is the "bla" gene and how does it relate to ampicillin resistance?

c. What component of the LB/AMP/ARA media is selective? What is does it select for?

d. How is this visualized on the media?

2. The reporter gene on the pGLO plasmid is GFP.

a. What does "GFP" stand for?

b. What is the purpose of the reporter gene? What does it tell you about the organism?

c. How does arabinose regulate the expression of GFP?

d. What would you EXPECT to see on the +pGLO LB/AMP plates if there was NO arabinose?

e. What are some possible explanations to why you may see colonies growing on the +pGLO LB/AMP/ARA plates that are NOT glowing?

3. The -pGLO tube was plated on the LB/AMP/ARA media.

a. Why was there no growth on these plates?

b. What would growth indicate on these plates?

4. On the LB/AMP/ARA plates:

a. What indicates successful transformation? (How is this visualized?)

b. What indicates successful gene expression? (How is this visualized?)