Generation and use of a standard curve and serial dilutions

Assignment Help Chemistry

Reference no: EM13125458

Generation and use of a standard curve and serial dilutions
This lab provided a 100mM Glucose standard solution which you are required to dilute with water to give final concentrations of 0,0.4,0.8,1.2,1.6 and 2.0 mmol/L glucose in a final volume 0.5ml. You will then use those concentrations to generate a standard curve using a spectrophotometer in order to calculate the amount of glucose in two different patient samples. Here are the absorbance-measurements of the six tubes: tube 1:0,tube 2:0.310, tube3:0.611, tube4:0.938, tube 5:1.016, tube6: 1.021

This solution will address the following:
1)produce a table of dilutions
2)prepare a 10-fold diluted 'patient sample ' from the above data to give 0.5ml final volume.Perform the 'patient samples' in duplicate.Absorbance for each solution taken at 550nm.
3)Results were obtained by measuring absorbances for the 2patients at time=0 and time=2hours to check glucose tolerance. So, time 0/patient 1 absorb=0.855 and 0.876 and patient 2 absorb=0.782 and 0.788 then at time 2hours we got for patient 1 absorb=0.748 and 0.752 and patient 2 absorb.=0.710 and 0.692.(remember is duplicated) From all this data use EXCEL to construct standard curves and determine the concentrations of the 2patient samples
4)Convert the values obtained in mmol/L into mg/dL and explain how
5)Use WHO guidelines for the diagnosis of diabetes to interprete the patients results during this Glucose Tolerance Test.
6)What component in Lucozade(given as a glucose resource) may have an adverse effect on the results of the Glucose Tolerance Test?

Reference no: EM13125458

Questions Cloud

Probabilty that none of the five tested carburators faulty : Tests it and then puts it to one side. this is repeated 5 times. What is the probabilty that none of the five tested were faulty?

Film and theatre : Film and theatre rely on different technologies. Do you think special effects in film add or detract from the quality of a film? Has the introduction of CGI effects enhanced the quality of filmmaking or are the films of the past essentially better th..

Determine the amount of operating cash flows the company : A company provides services on account during the current year totaling $400,000. By the end of the year, $350,000 of this amount had been received on account from customers for services provided in the prior years. Determine the amount of operati..

Expression for the aggregate expenditure curve : We now incur shocks as provided in red font. Solve for a new expression of the consumption function and aggregate expenditure curve, solve for the new equilibrium output and add this new equilibrium point to both of your diagrams (label as point B..

Generation and use of a standard curve and serial dilutions : Generation and use of a standard curve and serial dilutions, This lab provided a 100mM Glucose standard solution which you are required to dilute with water to give final concentrations of 0,0.4,0.8,1.2,1.6 and 2.0 mmol/L glucose in a final volume..

Determine mean number of news shows watched : Now they need your help to determine if the mean number of news shows watched in your neighborhood is similar to the national mean.

Explain combustion reaction : Combustion reaction, A fuel has the following weight percentage composition, C = 52%, H = 11%, S = 0%, O = 9%, moisture = 13%, Ash = 15%

What was the pension expense for the year : The actual return on plan assets was $1 million although it was expected to be $6 million. On average, employees' remaining service life with the company is 18 years.

What is the density of lead : What is the density of lead (in ) if a rectangular bar measuring 0.500 in height, 1.55 in width, and 25.00 in length has a mass of 220.9 ?

User Account

All Pages