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1. Assume you are looking for a particular pathogen in a sample and you are using a highly selective medium, and so you make five 1:10 dilutions of the sample and you plate 0.2 mLs of each dilution. The following day you find that you have 80, 50, 25, and 1 colonies on plates 2, 3, 4, and 5. How many pathogens per mL do you have?
2. You have 1mL containing 10^8 cells and you need 2.5x10^7 cells. What do you do? (how many cells in each 0.1mL)
3. You have 10 mL of 10^6 cells/mL prep and you need 1mL of 10^7 cells. What do you do? Hint: you can se cetrifugation, but then you have to perform another step)
4. You are generating a vaccine prep that contains 10^8 microbes per mL and you need to know the ID50 of your prep. So you run a challenge study with 50 mice where you inoculate each mouse with 50 uL of the prep. Within 3 days you record the number of sick mice and you find that 30 of the mice are sick. How can you estimate the ID50, and what is that estimate? (use a ration)
Compare the divisions of the autonomic nervous system's effect on heart rate and stroke volume.how to Describe an action potential.
Critically explain the environment and outcome of the environmental problems, the strategies and factors which affect the environment and prevention of the environmental strategies with the graphical explanation.
Listing all the possible parent crosses for the ABO blood group. There must be 21 possible crosses.
explain which is a key component of the cytoskeleton.
How do plant and animal cells metabolize energy in complementary ways? What does this suggest about the flow of energy and the recycling of elements through living organisms? Could we think of any other similar relationships.
Explain the reverse transcription reaction (what are the components of the reaction? What are the possible primers for the reaction and how do they differ from each other?)
Approximately what percentage of the energy of glucose is transferred to storage in ATP as the result of the complete oxidation of glucose to CO and water in cellular respiration.
Describe the differences between an integral membrane protein, a Davison-Danielli peripheral membrane protein, and a protein in which alpha-helices would not be found.
In enzyme lab. Manganese Dioxide does not come from a living thing. Is it a catalyst (define catalyst)? Is it an enzyme? Describe. Why did we test the hydrogen peroxide with manganese dioxide.
Assume you are an ecologist studying the effects of acid precipitation on plant life. Explain an experiment you would perform in order to determine which plant species will be a useful indicator species for acid rain pollution.
Structure and based analysis of proteins
When glucagon binds to it is receptor on a liver cell, which one of the following sequences of events occurs to activate protein kinase A?
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