Generate novel cell line

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Reference no: EM131762777

Read the Following Article. Based on these articles, PROPOSE A NOVEL research idea which must be related to those articles.

("Fasting Metabolism" article + along wit implementing the c-myb gene).

You cannot paraphrase any of those 2 articles. PROPOSE A NEW RESEARCH PROPOSAL. (ie: speak about a cell line that has never seen c-myb gene. + role in fasting metabolism)

- proposal must include following:

1) Objectives (3 in total)
2) Rationalization
3) Specific Aims)
4) Detailed project

Detailed project is 2 pages.

In detailed project, techniques employed could be CRISPR-CAS9 to generate novel cell line, CHip sequencing to look at specific interations, PCR or ChTAP. as well., feel free to use whichever is appropriate.

Please ensure it is A NOVEL EXPERIMENT.

it must be properly done and incorporate recent genetic techniques such as ChIP-seq, potebtially crispr/cas9 to generate a new cell line. Pcr to study interactions and so forth. Please ensure proposal is novel and follows format.

?Proposal based on the basis of those articles. Essentially speak how myc-b gene can be used in new proposal after reading "fasting metabolism" article and background info article about "mybc gene".

Verified Expert

This a detailed proposal for NSERC Grant for identifying the role of FFAR4 signaling on fasting metabolism in murine carcinogenic invitro models. The file details about aim, objectives, literature review, methodology and few pitfalls and study impact.

Reference no: EM131762777

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Reviews

inf1762777

4/20/2018 6:19:07 AM

To Expertsmind, Thank you so much for your help. I certainly plan to continue using your services because overall, I have received great papers from your experts and based on them I got good grades always. Once again, thank you.

inf1762777

1/18/2018 4:42:12 AM

No worries, however it must be properly done and incorporate recent genetic techniques such as ChIP-seq, potebtially crispr/cas9 to generate a new cell line. Pcr to study interactions and so forth. Please ensure proposal is novel and follows format Proposal based on the basis of those articles. Essentially speak how myc-b gene can be used in new proposal after reading "fasting metabolism" article and background info article about "mybc gene". Thank you Thank you I will wait until 5th I am currently checking with my supervisor, will let you know shortly for cell line vs. primary cells. As for the proposal, the "metabolism fasting" paper will be the starting point/basis of your project. You cannot paraphrase that article, rather you must propose a NOVEL RESEARCH PROECT which can speak about anything in particular, BUT must be related to the "metabolism fasting" article. DISREGARD MYB-C PAPER will provide more details later for cell vs. cell lines.

inf1762777

1/18/2018 4:35:07 AM

("Fasting Metabolism" article + along wit implementing the c-myb gene). You cannot paraphrase any of those 2 articles. PROPOSE A NEW RESEARCH PROPOSAL. (ie: speak about a cell line that has never seen c-myb gene. + role in fasting metabolism) - proposal must include following: 1)Objectives (3 in total) 2)Rationalization 3) Specific Aims) 4) Detailed project * 1-3 is 1 page. Detailed project is 2 pages. I do not want it double spaced I want it single spaced. 3 pages. I know it is possible since it has been done in previous assignments. Thank you I will submit the payment around noon (Eastern Time). Currently in class. PLease have the project done appropriately. This is worth 50% of final mark. I would like to achieve at least an 85% on the assignment. In detailed project, techniques employed could be CRISPR-CAS9 to generate novel cell line, CHip sequencing to look at specific interations, PCR or ChTAP. as well., feel free to use whichever is appropriate. I can wait until tomorrow. Please ensure it is A NOVEL EXPERIMENT. old experiments use + plagiarism is automatic 0%. ok is it being processed?

inf1762777

1/12/2018 1:44:12 AM

Will provide more details later for cell vs. cell lines. Kindly let me clear few queries: 1. What is the duration of your research project? 2. I search and found that we can elucidate the role of epigenetic enzymes in fasting metabolism and also as your mentioned earlier techniques can be employed for this work. Kindly let me know so that I can proceed with research proposal writing. Please employ cell line as we unsure what predicts the molecular signalling pathways and would seek to do so in a novel research proposal. The detailed proposal is 2 pages. The objectives/goals(1), rationalization, specific aims altogether is 1 page. Could use crispr with appropriate flagging antibody to generate the novel cell line that can be compared to cell line in which a transcription factor or molecule causes fasting metabolism as you stated for instance. Single spaced. You need to specify which epigenetic enzyme exactly. literature associated with it(ie: is it a transcription factor? What role in the signalling pathway, etc).

inf1762777

1/12/2018 1:39:25 AM

Please ensure it is A NOVEL EXPERIMENT. old experiments use + plagiarism is automatic 0%. ok is it being processed? No worries, however, it must be properly done and incorporate recent genetic techniques such as ChIP-seq, potentially crispr/cas9 to generate a new cell line. Pcr to study interactions and so forth. Please ensure the proposal is novel and follows format. A proposal based on the basis of those articles. Essentially speak how my-b gene can be used in the new proposal after reading "fasting metabolism" article and background info article about "mybc gene". Thank you I am currently checking with my supervisor, will let you know shortly for cell line vs. primary cells. As for the proposal, the "metabolism fasting" paper will be the starting point/basis of your project. You cannot paraphrase that article, rather you must propose a NOVEL RESEARCH PROJECT which can speak about anything in particular, BUT must be related to the "metabolism fasting" article. DISREGARD MYB-C PAPER

inf1762777

1/12/2018 1:35:34 AM

("Fasting Metabolism" article + along with implementing the c-my gene). You cannot paraphrase any of those 2 articles. PROPOSE A NEW RESEARCH PROPOSAL. (ie: speak about a cell line that has never seen c-myb gene. + role in fasting metabolism) - the proposal must include following: 1)Objectives (3 in total) 2)Rationalization 3) Specific Aims) 4) Detailed project * 1-3 is 1 page. The detailed project is 2 pages. I do not want it double spaced I want it single spaced. 3 pages. I know it is possible since it has been done in previous assignments. Thank you I will submit the payment around noon (Eastern Time). Currently in class. PLease have the project done appropriately. This is worth 50% of final mark. I would like to achieve at least an 85% on the assignment. In detailed project, techniques employed could be CRISPR-CAS9 to generate novel cell line, CHip sequencing to look at specific interations, PCR or ChTAP. as well., feel free to use whichever is appropriate.

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