Generate an in vitro system for the formation of copii

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Q. Your friend is trying to generate an in vitro system for studying the formation of COPII vesicles. He finds that to get well-organized vesicle budding from artificial membranes, he needs to add the coat proteins, the Sar1 GTP-binding protein, the GTPase-activating protein (GAP) for Sar1, and GMP-PNP (a nonhydrolyzable analog of GTP) to synthetic membranes; his experiment will not work in the presence of GTP. Give details why your friend needs to add GMP-PMP instead of GTP.

Reference no: EM135881

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