Gel filtration column with unknown matrix

Assignment Help Biology
Reference no: EM1386108

Question: Assume you have a gel filtration column with unknown matrix. Describe how do you experimentally deteremine its void volume and exclusion limit (experimentally means what you are going to do, step by step).

Reference no: EM1386108

Questions Cloud

Inversion or translocation heterozygotes : Inversion or Translocation heterozygotes have difficultyin producing gametes that function properly. Is there a way that an Inversion heterozygote could manufacture functional gametes?
Write jvm program which accepts rpn expression : Write down the IJVM program which accepts RPN expression, with each operand and operator entered line by line. As each number (operand) is entered, push it on stack.
Presence of hemoglobin in the catheter bag : Think about the presence of hemoglobin in the catheter bag. What symptom would this loss of hemoglobin most strongly and immediately be linked to in the patient?
Problem on throwing a die on ludo : If a six appears for the second time, the process is repeated with the move depending on the number shown and the previous throws. If a six appears for the third time, the turn of the player ends without moving the token.
Gel filtration column with unknown matrix : Assume you have a gel filtration column with unknown matrix. Describe how do you experimentally deteremine its void volume and exclusion limit
Find the void volume for the column : Gel filtration column was calibrated with blue dextran, its elution volume found to be 17.4 mL. Then, unknown protein and BSA were ran through the same column and eluted in 43 mL and thirty-six mL correspondingly.
Create shell script to enter length and width of rectangle : Network client needs you create the shell script which permits the user to enter length and width of rectangle. Perimeter of rectangle is then computed and printed to computer screen.
Winding stairs in jeromes apartment : What biological structure does the winding stairs in Jerome's apartment resemble and what components are represented by the "rails"?
Distinguish object frameworks-components-system installation : Distinguish object frameworks and components in terms of ease of modification before system installation, ease of alteration after system installation, and overall cost savings from code reuse.

Reviews

Write a Review

Biology Questions & Answers

  Immune response to infectious disease

It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.

  A review on advanced glycated end products (ages)

This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.

  Plastic as a soil stabilizer

Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.

  Principles of microbiology

This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.

  List the biologic functions

Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?

  Biologic function relationships

Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.

  Case study on patient in the haematology laboratory

Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.

  Use of pcr and genetic approaches in biotechnology

The use of PCR and genetic approaches in biotechnology

  Describe the role of this enzyme in honey

Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.

  Genetic problems

What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?

  Prepare an essay on nosocomial infection

Prepare an essay on nosocomial infection.

  Monitoring and recording the blood pressure

To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd