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1. If the lac operon is repressed in the absence of lactose, why do E. coli cells have small amounts of lactose permease and beta-galactosidase present at all times? What would happen to E. coli's ability to metabolize lactose if the LacI repressor protein was 100% efficient at blocking transcription of the operon?
2. How does the presence of lactose in an E. coli cell lead to derepression of the lac operon? What enzyme is involved? What is allolactose?
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