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Fusion proteins are produced by
A. replacing a portion of the gene with a synthetic sequence containing an altered DNA sequence.
B. recombination of two proteins in the bacterial cell to form a new hybrid protein.
C. introducing into a gene specific mutations that cause the expressed protein to fuse to other proteins.
D. ligating portions of two different genes to make a new hybrid gene. The hybrid gene product is a fusion protein derived from the two parent genes.
E. removing a portion of the gene to form a shorter gene and therefore a shorter protein.
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Calculate the following using complete solutions and indicating proper units.
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