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You have successfully purified a protein to homogeneity. From gel permeation chromatography studies, you determine that the protein has a molecular weight for 235,000 daltons. When you run the protein on a SDS PAGE gel you detect the presence of three protein subunits. The α subunit has a molecular weight of 50,000 daltons, the ß subunit has a molecular weight of 15,000 daltons and the γ band has a molecular weight of 5,000 daltons. From this data determine the number of α, ß and γ subunits present in the native protein.
Show all the steps in the mechanism for the following reaction, When benzene is mixed with deuterated sulfuric acid, deuterium is slowly incorporated onto the ring. Show the mechanism for this reaction and explain how this relates the sulfonation of ..
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