From this data determine the number of a, ß and ?

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You have successfully purified a protein to homogeneity. From gel permeation chromatography studies, you determine that the protein has a molecular weight for 235,000 daltons. When you run the protein on a SDS PAGE gel you detect the presence of three protein subunits. The α subunit has a molecular weight of 50,000 daltons, the ß subunit has a molecular weight of 15,000 daltons and the γ band has a molecular weight of 5,000 daltons. From this data determine the number of α, ß and γ subunits present in the native protein.

Reference no: EM13117901

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