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Rifamycin and actinomycin D are two antibiotics derived from the bacterium Streptomyces. Rifamycin binds to the b subunit of E. coli RNA polymerase and interferes with the formation of the first phosphodiester bond in the RNA chain. Actinomycin D bind to double stranded DNA by intercalation (slipping in between neighboring base pairs).
a) Which of the four stages in transcription would you expect rifamycin to affect primarily? Explain why.
b) Which of the four stages in transcription would you expect actinomycin D to affect primarily? Explain why.
c) Which of the two inhibitors is more likely to affect RNA synthesis in cultured human liver cells? Explain why.
d) Which of the two inhibitors would be more useful for an experiment in which it is necessary to block the initiation of new RNA chains without interfering with the elongation of chains that are already being synthesized? Explain why
How to write a discussion section for my electroencephalogram (EEG) lab report when all charts and data are already present.
If we have a dsDNA molecule and we require an ssRNA molecule, which of the two DNA strands will have the same sequence as the RNA molecule?
What is the significance of adenine, thymine, guanine, and cytosine in the structure of a DNA molecule.define how these four nucleotides bond.
The cell walls of bacteria, fungi, and plant cells and the extracellular matrix of animal cells are all external to the plasma membrane. Which of the following is a characteristic of all of these extracellular structures.
The brown and scarlet mutations assort independently. If fruit flies that are heterozygous for both of these mutations are intercrossed, what sorts of progeny will they create, and in what proportions? Describe how you got the answer.
Animals obtain energy during a sequence of chemical reaction in which sugar (C6H12O6) and oxygen gas (O2) are reactants. This process produces water (H2O) and carbon dioxide (CO2) as waste products. How might you use radioactive isotope to find out w..
How is geological energy different from biological energy. Charlie has the blood test that shows a normal level of LDLs other than an elevated level of HDLs in his blood. Because his family has a history of cardiovascular disease, he wonders if he mu..
10) The rhomboid muscles are innervated by (the) A) Dorsal scapular nerve. B) Cervical nerves. C) Accessory nerve. D) Long thoracic nerve. E) None of the above.
A 2-amino-purine can be incorporated into DNA in place of adenine. It can pair equally well with thymine or cytosine. What type of mutation do you expect it to cause.
Cut squares of developed film measuring 0.5cm x 0,5cm as accurately as you can. Thirty squares should be sufficient for the experiment.define Limitations of the experiment and improvements.
Eukaryotic genes differ than prokaryotic genes. Please define, in depth, the post-transcriptional modification that a eukaryotic mRNA goes through before it leaves the nucleus and how this differs from the transcriptional events in a prokaryotic s..
What are the steps in isolating the DNA from strawberries. What are the materials required to isolate DNA.
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