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A biologist was testing E. coli for thymidine requirement. She found a thy- point mutant and wanted to study reversion to thy+. She grew 20 cultures of the thy- mutant strain and plated them onto 20 plates that contained media to select for thy+ revertants. She obtained a total of 460 colonies. Six of the plates showed no colonies.a) If 5 x 108 cells were spread on each plate what was the reversion frequency and the reversion rate?b) Explain what steps she could have taken to find the thy- mutant in the first place, and why she did each step.c) She also wanted to study the type of mutation that occurred during formation of the original thy- cells. The mutagens HA (a base modifyer), BU (a base analogue), and acridine (an intercalator) were each added to thy- cells. Only BU reverted the thy- mutants to WT. Briefly describe the types of mutations each mutagen causes. Based on this information what was the original mutation to thy- to begin with? Explain your reasoning.
Assume you decide to make a by placing the promoter region from the Trp operon upstream of the coding region for the enzymes responsible for lactose metabolism.
For an amino acid such as alanine, the major species in solution at pH 7 is the zwitterionic form. Suppose a pKa value of 8 for the amino group and a pKa value of three for the carboxylic acid.
Suppose that you start with a lysogenic strain of E. coli. In this strain, a mutation arises that results in the intracellular repressor level becoming twice as high as it normally would be.
For a particular assay, you need cells at a concentration of 300 cells per ml. In your flask of cells growing you counted a sample using a hemacytometer.
A analyzer discoveres that the correlation coefficient between the exposure to a dangerous chemical and the growth of a certain species of butterfly is -.40.
Discuss how the unique physical and chemical properties of water contribute to the importance of water for life on Earth to survive.
There are a many respiratory diseases that can affect a person's ability to maintain a healthy respiratory system.
What is the use of having restriction enzyme sites at the 5' beginning of the luciferase gene and a set of restriction enzyme cut sites at the 3' end of the luciferase gene.
Determine what might be signified if the capillary refill time measures ten seconds in the digits of one had and 2 seconds in the digits of the other hand?
A population of cats can be either black or white; the black allele has complete dominance over the white allele. Given a population of 1,000 cats.
What is the genotypic and phenotypic ratio in the offspring produced by the cross AABb x Aabb if A is green colour, a - yellow; B- tall plants. b -short plant? Suppose complete dominance for the trait. Describe results of the cross using branching di..
How do Darwin's concepts of evolution and natural selection help explain the diversity of life on Earth today?
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