Reference no: EM132610702

Question 1. Highly expressed highly conserved gene: Gapdh
a) Paste the snapshots of Gapdh (using View>PDF) from human hg19, mouse mm10, fruit-fly, worm and yeast
b) Arrange Gapdh gene from each species based on its gene length
c) How many transcript isoforms of Gapdh are found in humans and mice
d) For Gapdh in humans, indicate the 5' and the 3' end of the gene.
e) Which is the least conserved exon of Gapdh across 100 vertebrates?
f) Which part of the Gapdh gene is not occupied by histones.
g) Enter the coordinates of the first exon of Gapdh and use "View > DNA" generate its DNA sequence. Find the position of the start codon within the DNA sequence.

Question 2. Highly expressed species specific gene: Myoglobin in human hg19
a) Which 3 tissues have high expression of this gene?
b) What is the minimum number of start codons that will be used by myoglobin transcripts?
c) Give the chromosome location for this gene in terms of chromosome number, chromosome loci such as p or q and the exact coordinates on the chromosome where the gene lies?

Question 3. Find in which tissues the following genes have the highest expression and what is the value of this expression.
a) Albumin
b) PCP2
c) Insulin preprotein (INS)

Question 4. Find which tissues have a high expression of TERT and TERC gene. Explain why?