Find the void volume for the column

Reference no: EM1386107

Question: Gel filtration column (60 mL) was calibrated with blue dextran, its elution volume found to be 17.4 mL. Then, unknown protein and BSA (MW 66 kDa) were ran through the same column and eluted in 43 mL and 36 mL correspondingly.

a) what is the void volume for the column?

b)which one has a higher molecular weight: unknown protein or BSA?

c) what is Kd for unknown protein?

Reference no: EM1386107

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