Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
RNA was extracted from plasma cells (PC) of wildtype (WT) mice and plasma cells (PC) from mice expressing a Myc short hairpin RNA (myc mutants). WT PC RNA is reverse transcribed in the presence of Cy3 dCTP (red) and myc mutant PC RNA is reverse-transcribed in the presence of Cy5 dCTP (green). Afterwards, both WT and myc mutant cDNA's are hydridized to a mouse microarray chip.
(1) At the end of this experiment, what color is the spot containing the Myc gene?(2) What color spots correspond to genes that are NOT regulated by Myc?
(3) Many genes can be down-regulated by JQ1. What color would most of the spots corresponding to JQ1-down-regulated genes be in this experiment?
(4) If you wanted to verify that the myc mutant mice had changes in production of Myc protein, what experiment would you do? Describe briefly in 1- 2 sentences.
You have developed a small molecule compound (called iHOPE ) that you hope activates a histone deacetylase that acts specifically on the chromatin near the Myc gene. Design a microarray experiment to test your hypothesis. Make sure your answer includes " If, then " sentences that describe how you will draw your conclusions.
Some wines are marketed as "sparkling" wines. Sparkling wines are considered so because of a dissolved gas that makes them fizzy. Concern what you know about the procedure of respiration to explain the type and origin of this gas.
make a discussion on the feasibility of using methylation analysis
A group of Ethiopians were assayed for CCR5-del32 deletion polymorphism. 223 were +/+, 64 were +/del32, and 6 were del32/del32.
define a well-controlled experiment to determine if the rise in internal free calcium observed in the cytoplasm of unfertilized sea urchin eggs in the presence of A23187 is due to in flux of calcium from seawater. Include experimental treatments, ..
Develop the process of transcription using dsDNA as a template to produce a final functional mRNA. In your drawing label the direction of the nucleic acids, RNA polymerase,
Create the action potential of a skeletal muscle cell and the action potential of a ventricular myocyte. Be sure to label the phases and the ions that are mediating the change in electrical potential.
The unfenced control plot had not changed significantly in species composition. By means of the concepts discussed in the chapter, what do you think happened.
Rifamycin and actinomycin D are two antibiotics derived from bacterium Streptomyces. Rifamycin binds to b subunit of E. coli RNA polymerase and interferes with formation of the first phosphodiester bond in the RNA chain.
Explain the prevalence of the cervical cancer in 1999 and 2004? Specify how many women were at the risk of developing the cervical cancer before being screened in: 1999, and 2004.
How many grams of methane should be combusted to heat 1.30 of water from 24.0 to 92.0, assume as product and 100 percent efficiency in heat transfer.
You wish to fuse a cDNA sequence to the end of two tandem"HA" antibody epitope tags within the MCS-HAHA region of the vector given below.
The 95 percent confidence interval for the relative risk of cardiovascular disease in the hormone group was 1.09 - 1.36
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd