Find the protein concentration in a fraction

Reference no: EM131383991

Determine the protein concentration in a fraction saved during cytochrome c purification. the Bradford method and used the standard BSA to create a standard curve. 10 µL from the original sample was taken and diluted to a total of 1000 µL total. Using 5 µL from the diluted solution and adding enough water and the dye to bring the total volume up to 3.0 mL.the absorbance value of a solution at 595nm was 0.060. What is the protein concentration (mg/mL) in the original solution? The equation from the BSA standard curve is y=0.0264x+0.0129 where y is the absorbance and x is the concentration in µg/mL

Reference no: EM131383991

Questions Cloud

What is the probability of either x or y occurring : The eventsXandYare mutually exclusive. SupposeP(X)_.05 andP(Y)_.02. What is the probability of either X or Y occurring? What is the probability that neitherXnorYwill happen?

Which of the following is true about a crosstab query : A function used to isolate a specific portion of a date such as year, month, or day in a date field is.Which of the following is true about a crosstab query?A function used to isolate a specific portion of a date such as year, month, or day in a date..

Which divides each line in the given ratio : Show that the lines AP, B Q, C R, DS all meet in a point (called the centroid of the tetrahedron), which divides each line in the ratio 3:1.

Give the answer of muliple choice question : The Acme Products case study notes that the senior management at Acme is concerned because the company takes so long to bring new products to the market. Which of the following approaches would your trainees identify as the most likely solution to..

Find the protein concentration in a fraction : The equation from the BSA standard curve is y=0.0264x+0.0129 where y is the absorbance and x is the concentration in µg/mL

What is the purpose of sds in the cell lysis solution : What is the purpose of SDS in the cell lysis solution? What is the function of the alcohol solution?

Give the answer of muliple choice question : Red Resource Inc. and Coral Techno Inc. are in a dispute over a contract. They decide to use a form of alternative dispute resolution (ADR) to settle the dispute. Both the parties, along with their respective lawyers, meet to discuss the issue. Ho..

What does phenotypical feature actually manifested depend on : For an individual with a genotype formed of two different alleles that condition different varieties of the same phenotypical trait, what does the phenotypical feature actually manifested depend on?

Give the answer of muliple choice question : Which of the following recruitment scenarios provide evidence of discrimination according to the four-fifths rule?The management of Carlson Meyer Consulting believes that married women with children are more likely to remain absent from work when co..

User Account

All Pages