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Q. You are working in a human genetics laboratory that studies causes and treatments for eye cataracts in newborns. This disease is thought to be caused by a deficiency in an enzyme called galactokinase, but the human gene that encodes this enzyme has not yet been identified. At a talk by a visiting scientist, you learn about a strain of bakers' yeast that contains a mutation called gal1- in its galactokinase gene. Because this gene is needed to metabolize galactose, the mutant strain cannot grow in galactose medium. Knowing that all living things evolved from a common ancestor and that distantly related organisms often have homologous genes that perform similar functions, you wonder if the human galactokinase gene can function in yeast. Because you have an optimistic temperament, you decide to pursue this line of experimentation. You isolate mRNA gene transcripts from human cells, use reverse transcriptase to make complementary DNA (cDNA) copies of the mRNA molecules, and ligate the cDNAs into circular plasmid DNA molecules that can be stably propagated in yeast cells. You then transform the pool of plasmids into gal1- (gal1 minus) yeast cells so that each cell receives a single plasmid. What do you think will happen when you spread the plasmidcontaining cells on Petri plates that contain galactose as a carbon source? How could this approach help you find the human gene encoding galactokinase?
Which of the follow statements regarding cancer risk factors is false. Mutagens are generally not carcinogens.
How did the rmp change with the low na ecf solution. Discuss at least three practical, scientific, and ethical issues that may be raised by such a change in naming.
Given an amount of sunlight that hits the plants on our planet, and ability of plants for rapid growth and reproduction, how come we are not all hip deep in dead plants.
While you measure the genotype frequencies of the population you get the following data. What will the frequency of Aa be in the next generation.
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He speculated that geologic columns would have the most height of 72,000 feet. Using his approach and numbers, compute the approximate age of the earth he came to.
Write down a detailed lab report on Separation and detection of proteins. Your report must include Title, Introduction, Materials and methods and three references for this report.
The Xerox machine becomes obsolete after five years. Compute the net present value for both scenarios and the profitability index for the buying scenario. What is the advice to the young entrepreneur? Suppose that funds have to be borrowed at an inte..
The trees derive no known benefits from the dwarf mistletoes but appear shorter than trees not associated with dwarf mistletoe. Which is the best describes the interactions between dwarf mistletoes and trees.
How can altering ice nucleation protein production yield a bacterial strain useful to agriculture? Explain the logic and how this works.
If a maize plant is heterozygous for the alleles for pigmy and crinkly-leaf (both recessive to normal size of plant (S) and normal leaf (L) is self pollinated and 208 seeds are subsequently collected and germinated, how various would you expect to sh..
What organism is most probable the cause of the symptoms. What is cause the man's disease? How is this disease transmitted? How may it be prevented.
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