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1. The recipe for 10x TBE buffer is 890 mM Tris, 890 mM boric acid, 20 mM EDTA. What is the concentration of 1x TBE buffer?2. A recipe calls for 10 ml of a 25mM stock of EDTA. However, all you have on hand is a 500 mM stock. How much more concentrated is what you have relative to what the recipe calls for? (i.e. what is the relative concentration).3. The protocol for transforming Chlamydomonas requires 300 ul of a culture at a density of 1x108 cells/ml. The culture you have is at 1x106/ml. What is the concentration of your culture relative to what the protocol calls for?
The PSA test is the most widely used test to detect prostate cancer. The PSA marker is a protease that is manufactured specifically in the prostate gland.
Suppose that you are member of a marine biology team seeking to catalogue the organisms inhabiting a threatened coral reef. The team has found two new seaweeds,
If the genotype of an organism is YySsTt, then yST would represent. the genotype of the offspring. a gamete of that parent organism. a possible phenotype of the offspring.
Oxygen is the final electron acceptor of this pathway. Glycolysis is the only energy yielding step of this pathway.
define how the rate of many enzyme catalyzed reactions varies with pH. What types of amino acids must be at the active site of an enzyme if its rate decreases at high pH.
A botanist studying waterlilies in an isolated pond observed 3-leaf shapes in the population, round, arrowhead, and scalloped.
A blue-eyed man marries a brown-eyed woman; and they have six children, all brown-eyed. What is the most likely genotype of the father?
A scientist has discovered a new cell. illustrate, in detail, ALL the ways the scientist could decide if the cell comes from a plant or an animal. How several of these result in the release of oxygen into the atmosphere?
What series of nucleotide pairs in a Drosophila gene will encode the amino acid sequence met-trp-phe-trp-met (reading from the amino terminus to the carboxyl terminus).
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Explain how this classification scheme presents different conclusions about the relationships among living organisms than those presented by the previous five-kingdom system of classification.
A plasmid digest with BamHI produced 2 kb and 4 kb fragments. Digest of a plasmid with HindIII produced 6 kb fragment. Double digest with both enzymes produced 1 kb, 2 kb and 3 kb fragments. Sketch a plasmid map.
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